FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492377975
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG21282420.4322374492888314TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3506300.095.4699861
TCGGAAG3624200.092.407192
CGGAAGA3928150.085.236133
AGAGCAC6621900.050.9729357
AGCACAC6954850.048.376439
GAGCACA7116400.047.3546148
GAAGAGC7323450.046.1117445
AAGAGCA10029400.033.9899676
GGAAGAG11061850.030.9107654
TATGCCG2844550.024.04757145-49
CTCGTAT2895750.022.98878540-44
CGTATGC2984350.022.63750545-49
TCTCGTA2954350.022.54505740-44
GTCACGA3140350.021.78492525-29
ATGCCGT3183600.021.52476745-49
TCGTATG3078900.021.328140-44
TGCCGTC3222500.020.96813845-49
GCCGTCT3251800.020.7992350-54
ATCTCGT3216000.020.7455140-44
ACGAGAT3315650.020.6702930-34
Produced by FastQC (version 0.11.5)