FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492377975
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC22066210.4481559111168609Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3658550.091.5662849
GAGCGTC4008450.083.676478
ATCGGAA4379800.077.2054141
AGAGCGT4440600.075.737127
TCGGAAG4504450.075.046832
CGGAAGA4784750.070.702473
AAGAGCG4981050.067.74226
GAAGAGC8236850.041.3934065
GGAAGAG12231600.028.2472554
CGCCGTA2994050.022.8039555-59
TCGCCGT3141700.021.71654555-59
GCCGTAT3230700.021.19907255-59
GTCGCCG3228550.020.96261255-59
TGGTCGC3392100.020.2206250-54
CCGTATC3392300.019.90798455-59
GGTCGCC3410850.019.83188850-54
CGTATCA3430250.019.7857760-64
GTGGTCG3530150.019.50010350-54
GCGTCGT3569100.019.0310810-14
CGTCGTG3622250.018.82347310-14
Produced by FastQC (version 0.11.5)