FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428854519
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC18724900.4366259225544036Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG7744200.038.6465079
GAGCGTC8242350.036.305528
AGAGCGT8682700.034.594387
AAGAGCG9291600.032.516866
TCGGAAG9506200.032.0130352
CGGAAGA9515900.031.9526943
ATCGGAA9592300.031.7661171
GAAGAGC12092700.025.2594345
GGAAGAG15629400.019.8766674
CGCCGTA3231300.018.60599155-59
TCGCCGT3388150.017.71940455-59
GCCGTAT3416300.017.64978855-59
CCGTATC3513400.016.97836555-59
GTCGCCG3561500.016.7730855-59
CGTATCA3566850.016.76766860-64
TGGTCGC3747650.016.1878150-54
GGTCGCC3746200.015.99747150-54
GTGGTCG3958700.015.37020550-54
TCTCGGT4545300.013.4705645-49
TCGGTGG4525100.013.40189145-49
Produced by FastQC (version 0.11.5)