FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404543471
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1034400.036.9892231
TCGGAAG1170050.032.849882
CGGAAGA1345200.028.5526493
TATGCCG627750.012.72730745-49
AGAGCAC3719000.010.9568027
CGTATGC743500.010.81219645-49
CTCGTAT691250.010.73426740-44
AGCACAC3997850.010.223469
TCTCGTA738000.010.14663440-44
GAGCACA4107600.010.0066968
GAAGAGC4163400.09.851695
GTCACGA852300.09.58715125-29
ATGCCGT896050.09.20123945-49
TGCCGTC880650.09.083878545-49
GCCGTCT922200.08.81865650-54
TCGTATG833200.08.78889440-44
ACGAGAT997500.08.34684730-34
ATCTCGT954050.08.04950140-44
AGTCACG1066400.07.848605625-29
CGTCTGA1092050.07.59073315-19
Produced by FastQC (version 0.11.5)