FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482021340
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG8947800.18563078555816637TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1674550.085.971711
TCGGAAG1823300.078.819572
CGGAAGA2053400.069.96933
AGAGCAC4964000.029.3810087
AGCACAC5366250.027.2488449
GAGCACA5448900.026.8488248
GAAGAGC5515400.026.5602975
TATGCCG1414700.020.72233245-49
TTACTCG1600450.018.74833530-34
CGTATGC1560500.018.6970345-49
CTCGTAT1489950.018.69055440-44
TCTCGTA1527400.018.25315940-44
AAGAGCA8232700.018.1283636
TGCCGTC1694200.017.73836145-49
TACTCGA1672200.017.53819335-39
ATGCCGT1718950.017.17428845-49
TCGTATG1663900.017.13552540-44
GCCGTCT1749900.016.69519250-54
GGAAGAG9237750.016.2367044
CGTCTGA1822600.016.15795315-19
Produced by FastQC (version 0.11.5)