FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442215140
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG16276310.3680631558657173TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2683150.0104.857911
TCGGAAG2814000.099.879852
CGGAAGA3046550.092.010143
AGAGCAC5621950.050.07127
AGCACAC5963750.047.253749
GAGCACA6067150.046.4950078
GAAGAGC6096650.046.3335275
AAGAGCA8682450.032.8006066
GGAAGAG9494650.030.1154544
TATGCCG2331000.024.2686945-49
CTCGTAT2245950.022.76522340-44
CGTATGC2484150.022.57874745-49
TCTCGTA2283700.022.3311340-44
ATGCCGT2608550.021.76597645-49
TGCCGTC2564950.021.74256545-49
GCCGTCT2606800.021.36691350-54
CCGGAGA2680650.021.00173830-34
ACTCCGG2716450.020.89951130-34
TCGTATG2417800.020.8268940-44
CGTCTGA2768000.020.58161715-19
Produced by FastQC (version 0.11.5)