FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464281157
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC85062061.832123891256694Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG10402700.0127.800929
GAGCGTC10776750.0123.2900548
ATCGGAA11070650.0121.271281
AGAGCGT11158550.0119.2107547
TCGGAAG11255400.0118.979232
CGGAAGA11494000.0116.371553
AAGAGCG11622300.0114.875216
GAAGAGC14556250.091.913795
GGAAGAG18414900.072.898444
CGCCGTA9506050.028.01527855-59
TCGCCGT9639450.027.56831455-59
GCCGTAT9743800.027.29479855-59
GTCGCCG9804550.027.0110755-59
TGGTCGC9956050.026.8215650-54
CCGTATC9833100.026.7356555-59
GGTCGCC9981200.026.54507450-54
CGTATCA9907200.026.48382460-64
GTGGTCG10166450.026.2744650-54
GCGTCGT10349050.025.80993310-14
CGTCGTG10375300.025.77216310-14
Produced by FastQC (version 0.11.5)