FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470840645
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG17143460.36410323072257283TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3201900.086.678411
TCGGAAG3348700.082.9475862
CGGAAGA3583000.077.465263
AGAGCAC6191850.045.054667
AGCACAC6458300.043.0532659
GAGCACA6605600.042.276518
GAAGAGC6751600.041.4793365
AAGAGCA9402800.030.0429296
GGAAGAG10525400.026.983964
TATGCCG2352150.024.05729745-49
CGGCTAT2453150.022.9552630-34
CTCGTAT2414100.022.63344240-44
CGTATGC2486650.022.434245-49
TCTCGTA2453250.022.27579540-44
ATGCCGT2646400.021.42458345-49
ACCGGCT2650850.021.29412530-34
TCGTATG2538600.021.12763440-44
TGCCGTC2646250.021.02581845-49
GCCGTCT2683850.020.84438950-54
TCACCGG2698150.020.5371125-29
Produced by FastQC (version 0.11.5)