FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432100263
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1247150.039.6676181
TCGGAAG1401350.035.3747562
CGGAAGA1591150.031.2234483
TATGCCG778550.014.69122245-49
CGTATGC898850.012.74111645-49
AGAGCAC4088850.012.7377187
CTCGTAT828950.012.40040340-44
AGCACAC4366800.011.9186539
TCTCGTA878600.011.71450340-44
GAGCACA4498000.011.6467468
GAAGAGC4596100.011.421355
GTCACGA1019450.011.35929925-29
ATGCCGT1062500.010.92469145-49
GCCGTCT1121650.010.24056950-54
ACGAGAT1150350.010.13117630-34
GATCGGA722300.09.9713291
ATCTCGT1082000.09.64366640-44
TGCCGTC1072000.09.52544945-49
AGTCACG1245250.09.41594725-29
CGTCTGA1281750.09.13561115-19
Produced by FastQC (version 0.11.5)