FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432100263
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1272300.038.4335679
GAGCGTC1633700.030.0459868
ATCGGAA1758250.028.3826681
TCGGAAG1903700.026.1978422
AGAGCGT1950500.025.387
CGGAAGA2150350.023.2310453
AAGAGCG2361650.021.0894666
CGCCGTA800150.014.15238455-59
TCGCCGT956600.011.96868555-59
GCCGTAT1036100.011.15451155-59
GTCGCCG1083950.010.53994855-59
GAAGAGC5075000.010.3457295
TGGTCGC1214600.09.60886550-54
CGTATCA1228250.09.53229460-64
GCGTCGT1204950.09.31434410-14
CGTCGTG1259050.08.94955410-14
CCGTATC1157100.08.90094555-59
GTGGTCG1320250.08.87264150-54
GGTCGCC1252300.08.23236550-54
TCGTGTA1497300.07.709110-14
Produced by FastQC (version 0.11.5)