FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456979447
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG22418680.49058398899940026TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3940900.097.1415561
TCGGAAG4115400.092.9846652
CGGAAGA4330800.088.315533
AGAGCAC6934600.055.345587
AGCACAC7177600.053.384069
GAGCACA7337650.052.3184248
GAAGAGC7523850.051.1334085
AAGAGCA9970950.038.8370976
GGAAGAG11236500.034.54534
TATGCCG3157450.024.93401545-49
CTCGTAT3057300.023.82396740-44
CGTATGC3291900.023.68662645-49
TCTCGTA3100350.023.45574640-44
TACTCGA3389750.022.94179535-39
ATGCCGT3450150.022.80188245-49
TTACTCG3353950.022.65220830-34
GCCGTCT3514100.022.043250-54
TCGTATG3214650.021.90958240-44
TGCCGTC3490850.021.80756645-49
ATCTCGT3343000.021.77317240-44
Produced by FastQC (version 0.11.5)