FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456979447
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC24459450.5352417961151762Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG4066700.094.618849
GAGCGTC4472100.086.039948
ATCGGAA4674850.083.949581
TCGGAAG4860750.080.365932
AGAGCGT4818550.080.112577
CGGAAGA5108550.076.336553
AAGAGCG5296450.073.298766
GAAGAGC8268400.047.3709225
GGAAGAG12142550.032.6755034
CGCCGTA3243350.023.90777855-59
TCGCCGT3381100.022.86600355-59
GCCGTAT3484100.022.34563355-59
GTCGCCG3603900.021.65873755-59
GTACGTC3674150.021.19200330-34
TGGTCGC3721050.021.15767950-54
CGTATCA3665100.021.09293460-64
CCGTATC3587800.021.00115455-59
TACGTCC3747450.020.9256830-34
GTGGTCG3891150.020.35982550-54
GGTCGCC3779050.020.27709850-54
Produced by FastQC (version 0.11.5)