FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459277419
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG14755260.3212711835937225TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3031800.084.153131
TCGGAAG3206900.079.5464252
CGGAAGA3410200.074.748993
AGAGCAC6016000.042.559127
AGCACAC6287050.040.6958549
GAGCACA6433950.039.86478
GAAGAGC6559950.039.198725
AAGAGCA9119650.028.4340926
GGAAGAG10293550.025.3984414
TATGCCG2241850.023.16498645-49
CTCGTAT2228600.021.78053940-44
CGTATGC2368750.021.61182845-49
TCTCGTA2275950.021.31084340-44
CGCTCAT2494000.020.59186630-34
ATGCCGT2527600.020.55302245-49
TATCTCG2365400.020.25916740-44
TCGTATG2379050.019.95218340-44
TGCCGTC2551100.019.88915645-49
ACCGCTC2580950.019.85657730-34
GCCGTCT2584100.019.69207450-54
Produced by FastQC (version 0.11.5)