FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459277419
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC16420560.3575303143741103Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3216200.079.389319
GAGCGTC3614350.070.716858
ATCGGAA3810850.068.101981
TCGGAAG3987650.064.952352
AGAGCGT3959950.064.730037
CGGAAGA4214450.061.4240383
AAGAGCG4429600.058.308926
GAAGAGC7388600.035.3906675
GGAAGAG11243500.023.6398124
CGCCGTA2388950.021.57464855-59
TCGCCGT2552700.020.17534455-59
GCCGTAT2644350.019.58904355-59
GTCGCCG2737250.018.90990455-59
CCGTATC2753050.018.40902555-59
GTACGTC2834050.018.2617130-34
TGGTCGC2866850.018.21706650-54
CGTATCA2830250.018.17420260-64
TACGTCC2911700.017.97188830-34
GGTCGCC2922100.017.55708550-54
GTGGTCG3040950.017.27183750-54
Produced by FastQC (version 0.11.5)