FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004963994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453757128
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT27721620.6109351961518938TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3793500.0119.7158051
ATCGGAA4527400.0100.602242
TCGGAAG4671800.097.416643
CGGAAGA4933600.092.320134
AGAGCAC7517000.060.594968
GAGCACA7911300.057.5648279
GAAGAGC8026600.057.0684626
AAGAGCA10539650.043.628267
GGAAGAG11616850.039.7842945
CGCGAAA3845400.027.10036335-39
TCCGCGA3870950.026.96443730-34
TATGCCG4032850.026.13463845-49
CGTATGC4165100.025.21465345-49
CTCGTAT3935850.025.18867940-44
TCTCGTA3976550.025.01729640-44
GCGAAAT4183000.025.00709535-39
CTCCGCG4203050.024.95977230-34
ATGCCGT4305050.024.32659345-49
ACTCCGC4326050.024.27725230-34
GCCGTCT4358900.023.91040450-54
Produced by FastQC (version 0.11.5)