FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004964004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004964004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492439377
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG6864670.13940132167781538TruSeq Adapter, Index 13 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1708650.065.083261
TCGGAAG1788550.062.129312
CGGAAGA2104650.052.7910043
AGAGCAC4767200.023.630017
AGCACAC5114800.022.124719
GAGCACA5249700.021.5520388
GAAGAGC5557050.020.5726645
TATGCCG1278450.017.841445-49
CGTATGC1422900.015.96905145-49
CTCGTAT1410500.015.59813540-44
TCTCGTA1457850.015.06765840-44
GCCGTCT1585350.014.37497950-54
TGCCGTC1591700.014.25185245-49
ATGCCGT1628250.014.21593345-49
AAGAGCA8320150.013.9870086
TCGTATG1589750.013.68531540-44
ACACGTC1708350.013.50276810-14
CGTCTGA1722750.013.3835415-19
ATCTCGT1704350.013.03043840-44
GGAAGAG9192800.012.8034944
Produced by FastQC (version 0.11.5)