FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004964007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004964007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483348872
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC8636490.17868025561462364Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1674450.078.353539
GAGCGTC2006650.065.5735868
ATCGGAA2232450.059.721271
TCGGAAG2350100.056.7347872
AGAGCGT2436650.054.171267
CGGAAGA2639650.050.5326083
AAGAGCG2852300.046.503366
TCCGATC576050.036.9821743
CCGATCT713400.028.6630924
TTCCGAT807300.026.7071652
CTTCCGA918650.023.7355121
GAAGAGC5999150.022.7000475
CGCCGTA1316900.020.5081755-59
TCGCCGT1434250.018.88174455-59
GTCGCCG1428550.018.75310155-59
GCCGTAT1603500.017.04518155-59
GTACGTC1617550.016.68509330-34
GCGTCGT1633750.016.49596810-14
TGGTCGC1669800.016.47321550-54
GGTCGCC1642400.016.2475350-54
Produced by FastQC (version 0.11.5)