FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004964012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004964012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491095977
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG77162431.571229120453577TruSeq Adapter, Index 3 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA10124500.0121.492491
TCGGAAG10415900.0118.193962
CGGAAGA10666500.0115.347763
AGAGCAC13286000.092.493237
AGCACAC13538600.090.454899
GAGCACA13732400.089.32988
GAAGAGC13940950.088.3650055
AAGAGCA16594800.074.309596
GGAAGAG17869000.069.213424
TATGCCG8997250.027.5066245-49
GCGCATC9095400.027.14375735-39
CTCGTAT8953400.027.05732540-44
TAATGCG9184050.026.9465830-34
TCTCGTA8995350.026.91019240-44
CGTATGC9156750.026.70816645-49
TGCGCAT9256400.026.64172235-39
ATGCCGT9301700.026.59088545-49
AATGCGC9242750.026.52746630-34
CGCATCT9153850.026.4353135-39
TCGTATG9103700.026.37303440-44
Produced by FastQC (version 0.11.5)