FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004964013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004964013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491095977
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC77553891.579200271070435Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG9876850.0123.813959
GAGCGTC10276800.0119.067398
ATCGGAA10476900.0117.53991
AGAGCGT10619900.0115.236787
TCGGAAG10780850.0114.096482
CGGAAGA11014950.0111.610573
AAGAGCG11126800.0110.2462166
GAAGAGC14320800.085.922585
GGAAGAG18294650.067.5559844
CGCCGTA8872250.027.7276455-59
TCGCCGT8990850.027.28755455-59
GCCGTAT9136300.026.96070755-59
GTCGCCG9169600.026.62930555-59
TGGTCGC9271500.026.5541750-54
CCGTATC9258150.026.35034255-59
GGTCGCC9330300.026.18141650-54
CGTATCA9351000.026.09413360-64
GTGGTCG9467400.026.04115350-54
TCTCGGT9839800.025.1945345-49
GCGTCGT9775150.025.10972210-14
Produced by FastQC (version 0.11.5)