FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004964015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004964015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474155984
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC11116950.2344576547619823Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2199100.078.652649
GAGCGTC2537100.067.9911048
ATCGGAA2624150.066.46821
TCGGAAG2790200.062.276772
AGAGCGT2939000.058.7748037
CGGAAGA3023700.057.526173
TCCGATC960850.055.6716773
TTCCGAT978100.054.9735372
AAGAGCG3306350.052.47926
CTTCCGA1141700.047.6112631
CCGATCT1119350.047.0111474
CGATCTA524700.033.451595
GAAGAGC6384050.027.711645
CGCCGTA1691100.020.6820255-59
TCGCCGT1814550.019.25657555-59
GTCGCCG1890400.018.49543455-59
GCCGTAT1970700.017.8889555-59
CCGTATC2089950.017.54742655-59
GGAAGAG10376200.017.4071454
GGTCGCC2124300.017.33217250-54
Produced by FastQC (version 0.11.5)