FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004964020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004964020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479306403
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG11224370.23417942947864187TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2868650.064.5810551
TCGGAAG3016200.061.54032
CGGAAGA3249450.057.2077373
AGAGCAC5924650.031.657657
AGCACAC6182100.030.338659
GAGCACA6331400.029.6678898
GAAGAGC6514300.028.9040135
TATGCCG1863600.021.8996245-49
AAGAGCA9172100.020.7872586
CGTATGC2010900.020.14909445-49
CTCGTAT1917600.020.09675440-44
TCTCGTA1966300.019.6189240-44
ATGCCGT2163350.018.9121645-49
TACTCGA2145600.018.88825835-39
GGAAGAG10361900.018.5694624
GCCGTCT2214100.018.28584350-54
TTACTCG2117450.017.56837730-34
ATCTCGT2214350.017.49847840-44
TGCCGTC2170550.017.16280645-49
TCGTATG2065800.016.95106940-44
Produced by FastQC (version 0.11.5)