FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004964022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004964022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463202971
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG9804450.21166638846968883TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2438900.063.4502561
TCGGAAG2570750.060.2864272
CGGAAGA2783400.055.6101343
AGAGCAC5470600.028.5025737
AGCACAC5817050.026.8511039
GAGCACA5925550.026.3594688
GAAGAGC6072150.025.9641595
TATGCCG1547400.020.34961745-49
CTCGTAT1643900.018.7817140-44
CGTATGC1689550.018.46588145-49
AAGAGCA8658700.018.4274126
TCTCGTA1695500.018.23320440-44
TTACTCG1796150.017.82392730-34
TCGTATG1799450.017.45223840-44
TGCCGTC1841850.017.45219445-49
ATGCCGT1831500.017.33708445-49
TACTCGA1820500.017.1342435-39
GCCGTCT1878400.016.70548850-54
GGAAGAG9808900.016.4343134
ATCTCGT1955200.015.88552440-44
Produced by FastQC (version 0.11.5)