FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004964023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004964023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463202971
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC9620340.2076916730311732Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2347650.064.831559
TTCCGAT1029150.060.318782
TCCGATC1091250.056.73483
GAGCGTC2707200.056.4376268
CTTCCGA1180950.052.609991
ATCGGAA2988650.051.846111
TCGGAAG3120500.049.647162
AGAGCGT3095400.049.6092957
CCGATCT1201400.048.1123164
CGGAAGA3330500.046.5332453
AAGAGCG3533250.043.7881246
CGATCTA577600.038.4392285
GAAGAGC6650550.023.7723945
CGCCGTA1490500.020.52158455-59
CGATCTG765050.019.4989155
TCGCCGT1589100.019.13694655-59
GTCGCCG1671400.018.37421855-59
GCCGTAT1744400.017.66763155-59
GGTCGCC1877100.016.95098750-54
CCGTATC1860250.016.842555-59
Produced by FastQC (version 0.11.5)