FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004964028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004964028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442896135
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG4568840.10315827208562116TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1491550.055.1268161
TCGGAAG1653350.049.789552
CGGAAGA1891550.043.5370563
AGAGCAC4401950.019.1749867
AGCACAC4673800.018.1104979
GAGCACA4841600.017.4991518
TATGCCG1045800.017.39477745-49
GAAGAGC4918250.017.2726885
CGGCTAT1101950.016.53718630-34
CGTATGC1175250.015.32335745-49
CTCGTAT1075050.015.29495940-44
TCTCGTA1127950.014.57892140-44
ACCGGCT1314150.013.95734130-34
ATGCCGT1324650.013.83919945-49
GTCACCG1427900.012.95183925-29
GCCGTCT1398000.012.91702950-54
TCACCGG1350550.012.365625-29
TGCCGTC1352850.012.33540245-49
ATCTCGT1362100.012.13661740-44
ACACGTC1536500.012.08135310-14
Produced by FastQC (version 0.11.5)