FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004964030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004964030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411567332
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG9017280.21909610649078434TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2312700.072.048311
TCGGAAG2421700.068.7640462
CGGAAGA2628800.063.313593
AGAGCAC4947900.033.976547
AGCACAC5228800.032.076859
GAGCACA5334650.031.5920818
GAAGAGC5440550.031.0260685
AAGAGCA7831600.021.8571976
TATGCCG1653400.020.54413445-49
GGAAGAG8773300.019.6465024
CGTATGC1760900.019.09811245-49
CTCGTAT1612650.018.79835940-44
TCTCGTA1647850.018.39944540-44
CGCTCAT1861700.018.02852230-34
ATGCCGT1892700.017.97884845-49
TGCCGTC1869000.017.70494345-49
TATCTCG1703200.017.55803540-44
GCCGTCT1905350.017.42813950-54
ACCGCTC1925750.017.39878330-34
GTCACCG2007500.016.96038825-29
Produced by FastQC (version 0.11.5)