FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004964031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004964031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411567332
Sequences flagged as poor quality0
Sequence length151
%GC40

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC10579150.257045425558703Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2320150.071.5597849
GAGCGTC2637350.062.983368
ATCGGAA2858250.058.708681
AGAGCGT2971000.056.0948527
TCGGAAG2992300.055.955452
CGGAAGA3224800.051.997043
AAGAGCG3400000.049.1896976
GAAGAGC5984550.028.4209145
CGCCGTA1701900.020.02342855-59
GGAAGAG9208700.018.8306674
TCGCCGT1827850.018.6627355-59
GCCGTAT1919950.017.86564855-59
GTCGCCG1953200.017.48208855-59
TGGTCGC2089950.016.54230750-54
CCGTATC2040550.016.39480855-59
CGTATCA2105200.016.23503160-64
GGTCGCC2118900.015.88862650-54
GTGGTCG2202750.015.73798150-54
GCGTCGT2242050.015.18914310-14
CGTCGTG2285400.014.93782310-14
Produced by FastQC (version 0.11.5)