FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004964032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004964032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465425482
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG19263910.4138989106745986TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3482200.086.321931
TCGGAAG3558850.084.357882
CGGAAGA3885200.077.2611853
AGAGCAC6213650.048.5698747
AGCACAC6537550.046.1688739
GAGCACA6661650.045.3164068
GAAGAGC7078500.042.832235
AAGAGCA9514400.032.02856
GGAAGAG10356300.029.5814364
TATGCCG2774850.022.12519345-49
CTCGTAT2809550.021.20559540-44
CGTATGC2889550.021.00510445-49
TCTCGTA2858350.020.84304840-44
TCGTATG2954750.019.86274740-44
ACTCCGG3140150.019.64515730-34
ATGCCGT3158200.019.53736345-49
CCGGAGA3118600.019.44386730-34
GCCGTCT3160850.019.2248950-54
ATCTCGT3136800.019.04090540-44
CTCCGGA3253250.019.03349130-34
Produced by FastQC (version 0.11.5)