FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004964033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004964033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465425482
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC19877740.427087488089017Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3406450.087.764979
GAGCGTC3830750.078.095088
ATCGGAA4055400.074.579921
TCGGAAG4141050.072.921242
AGAGCGT4266750.070.3341457
CGGAAGA4474100.067.5243453
AAGAGCG4774350.063.1426126
GAAGAGC7685550.039.66135
GGAAGAG11168150.027.662154
TCCGATC561500.023.5425763
CGCCGTA2805100.021.89002655-59
TCGCCGT3034350.020.24384555-59
GCCGTAT3064400.020.14232355-59
GTCGCCG3091650.019.68950555-59
CCGTATC3189550.019.06015455-59
TGGTCGC3266300.018.9688150-54
GGTCGCC3251550.018.77304550-54
CGTATCA3278300.018.59656560-64
GTGGTCG3371850.018.40727250-54
GCGTCGT3385200.018.03260610-14
Produced by FastQC (version 0.11.5)