FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004964036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004964036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439834526
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG135674723.0846764403392926TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA19354600.0111.6082151
TCGGAAG19674200.0109.7512362
CGGAAGA19754200.0109.2386553
AGAGCAC21785900.098.895117
AGCACAC21877750.098.31869
GAGCACA22092750.097.445788
GAAGAGC22513750.095.874665
AAGAGCA24801350.087.071216
GGAAGAG26092450.082.951784
TATGCCG16153900.026.8766645-49
CTCGTAT16124600.026.52763640-44
ATGCCGT16381450.026.45093745-49
TCTCGTA16223000.026.36717840-44
CGTATGC16339750.026.3106645-49
TGCCGTC16446700.026.20038445-49
TCGTATG16258250.026.13560140-44
CGTCTTC16578750.026.1080950-54
GCCGTCT16419900.026.10056950-54
CCGTCTT16627600.026.0084650-54
ATCTCGT16526600.025.89999640-44
Produced by FastQC (version 0.11.5)