FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004964037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004964037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439834526
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC133949423.0454503246523217Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG18520950.0115.568919
GAGCGTC18929700.0113.032928
AGAGCGT19360150.0111.169057
ATCGGAA19663050.0109.8070141
TCGGAAG19865150.0108.483782
AAGAGCG19891100.0108.157146
CGGAAGA19982600.0107.908973
GAAGAGC22751250.094.797095
GGAAGAG26346900.082.03794
CGCCGTA15473850.027.33889255-59
TCGCCGT15621550.027.04291755-59
GCCGTAT15644900.027.02488555-59
CCGTATC15748650.026.65024255-59
TGGTCGC16127450.026.5930250-54
GTCGCCG15911400.026.54063255-59
CGTATCA15880350.026.5266760-64
GGTCGCC16049050.026.3606150-54
GTGGTCG16465750.026.15564550-54
TCTCGGT16798550.025.67326445-49
CTCGGTG16955050.025.42303845-49
Produced by FastQC (version 0.11.5)