FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004964039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004964039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420891901
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC27535970.6542290297004314Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG6841200.063.3566559
GAGCGTC7229250.059.914768
AGAGCGT7642150.056.799037
ATCGGAA8150400.054.028751
TCGGAAG8182350.053.669932
AAGAGCG8170350.053.316056
CGGAAGA8254000.053.1552353
GAAGAGC10896350.040.305025
GGAAGAG14282850.031.131774
CGCCGTA4005500.021.98381255-59
TCGCCGT4131900.021.293455-59
GCCGTAT4194850.021.02641355-59
GTCGCCG4252900.020.5753855-59
CCGTATC4313500.020.19419155-59
CGTATCA4359800.020.08372960-64
TGGTCGC4444250.019.90747850-54
GGTCGCC4440750.019.638150-54
GTGGTCG4597600.019.28750-54
TCTCGGT5075650.017.57053845-49
TCGGTGG5080950.017.33905645-49
Produced by FastQC (version 0.11.5)