FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004964042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004964042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407100525
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG8318650.20433896517328246TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1938400.069.594291
TCGGAAG2081800.064.963582
CGGAAGA2220450.060.858093
AGAGCAC4525800.030.1737127
AGCACAC4798250.028.498189
GAGCACA4904150.027.9271328
GAAGAGC5066650.027.211735
TATGCCG1333150.020.74852445-49
CGGCTAT1402050.019.742830-34
CTCGTAT1407750.019.16633640-44
AAGAGCA7310800.019.118466
CGTATGC1443400.018.94974945-49
TCTCGTA1446850.018.6283440-44
ACCGGCT1587550.017.52450430-34
ATGCCGT1603000.017.39951745-49
GGAAGAG8223150.017.1330534
TCGTATG1569700.016.86192740-44
TGCCGTC1620200.016.84074445-49
TCACCGG1629200.016.64964725-29
GCCGTCT1656900.016.6174550-54
Produced by FastQC (version 0.11.5)