FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004964044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004964044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446269481
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG110471722.4754486852306172TruSeq Adapter, Index 13 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA22487550.077.903611
TCGGAAG22514600.077.7824552
CGGAAGA22689800.077.239133
AGCACAC23831650.073.2750249
AGAGCAC23957050.073.085397
GAGCACA24123750.072.569848
GAAGAGC25298100.069.345985
AAGAGCA27342800.064.2038356
GGAAGAG28448150.061.8278924
TATGCCG15411650.022.86921145-49
ATGCCGT15687500.022.46236645-49
CGTCTTC15671150.022.4237950-54
GCCGTCT15608150.022.35343250-54
CGTATGC15660200.022.34542745-49
CCGTCTT15750950.022.30622750-54
CTCGTAT15575750.022.24467740-44
TGCCGTC15653500.022.23161545-49
TCTCGTA15747500.022.02434240-44
TCGTATG15626850.021.92222640-44
ATCTCGT16119700.021.52542540-44
Produced by FastQC (version 0.11.5)