FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004964049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004964049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475848121
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC70192601.475105120778653Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG10699200.0102.27172
ATCGGAA10856750.0100.943641
GAGCGTC11092950.098.176938
AGAGCGT11122850.098.116527
AAGAGCG11638200.093.94496
AGCGTCG11812800.092.310629
CGGAAGA12425600.088.142443
GAAGAGC14522050.075.5129245
GGAAGAG17384300.063.2490584
CGTATCA9185650.023.77887560-64
GCCGTAT9563800.023.0679155-59
TCGTGTA9544350.022.995710-14
TCTCGGT9660150.022.89704745-49
CCGTATC9555250.022.82997955-59
GTCGTGT9746550.022.53797510-14
CGTGTAG9695350.022.48800810-14
CTCGGTG10062500.022.01291845-49
GTGGTCG10083550.021.9556650-54
GTAGATC10132100.021.87484240-44
TCGGTGG10299100.021.30464245-49
Produced by FastQC (version 0.11.5)