FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004964429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004964429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39485522
Sequences flagged as poor quality0
Sequence length15-42
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCTTA107050.086.1610826
CTTACTA108200.085.2260429
TTACTAC108750.084.95293430
ACCTTAC108950.084.93304427
TACTACC115600.079.9012831
CCTTACT115950.079.78342428
ACTACCA118000.078.1911832
CCACCTT129950.071.16257525
CTACCAG136050.068.2224233
TCCACCT142150.064.9573724
TACCAGA150850.062.75732834
TTAGACG12800.062.74971834
ACCAGAC152950.061.83162735
ACTCCAC128000.061.23647722
CCAGACA163600.058.8753736
ACGAGTA13900.058.03679727
ACTATTA13400.057.0075536
TTCGGAT83250.054.7622728
TCGGATC87550.051.87710629
ATCATGT88600.051.59536733
Produced by FastQC (version 0.11.5)