FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004964430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004964430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27206201
Sequences flagged as poor quality0
Sequence length15-42
%GC65

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCAGA241750.060.06683334
TTACTAC235300.060.0603530
CACCTTA234250.059.74953526
ACCTTAC235800.059.7356527
CTTACTA236900.059.58394229
CCTTACT241800.058.45767228
ACTACCA243750.057.9412432
TACTACC246150.057.448631
TATCTAG25500.056.80370336
CTACCAG254550.055.77361333
CCACCTT253350.055.08821525
CTATCTA26450.055.01242435
CCAGACA268350.054.55439436
TCCACCT259850.053.5994424
ACTCCAC252000.050.9598422
ACCAGAC292000.049.51578535
TCTATCT30350.048.16021734
CTCCACC292700.047.45394523
TTAGACG9100.043.77318634
ATAGGCG36250.040.77580336
Produced by FastQC (version 0.11.5)