FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004964441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004964441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39991768
Sequences flagged as poor quality0
Sequence length15-42
%GC64

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCTTA153500.079.0330926
TTACTAC154200.078.6824630
CTTACTA154100.078.51298529
ACCTTAC154750.078.4514827
TACTACC159250.076.53448531
CCTTACT159800.076.0218228
ACTACCA163950.074.2898232
CCACCTT175650.068.8072225
CTACCAG181800.067.44133
TACCAGA192000.064.96474534
TCCACCT189950.063.63274424
ACTCCAC179050.060.3375422
ACCAGAC207750.059.9761535
CCAGACA221750.057.0725936
ACGAGTA13450.056.53411527
CTCCACC235350.051.69643423
ACTATTA16900.050.02976636
GCTACAT37450.048.0055235
CTACATC39650.045.1174436
CCAGCAT82250.044.52721435
Produced by FastQC (version 0.11.5)