FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004964443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004964443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37816255
Sequences flagged as poor quality0
Sequence length15-42
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTAC167800.081.32683630
CACCTTA168100.081.10612526
ACCTTAC169450.080.7191827
CTTACTA169300.080.61194629
CCTTACT175300.078.07228
TACTACC174900.077.9083831
ACTACCA179000.076.1553932
CTACCAG190250.072.0655433
CCACCTT189600.071.9211225
TCCACCT197950.068.55662524
TACCAGA209100.066.9582534
CCAGACA211600.066.7660436
ACCAGAC216900.064.5703335
ACTCCAC189550.062.29568522
ACTATTA19250.061.96382536
TTAGACG11500.060.7357534
ACGAGTA18950.060.20680627
CTCCACC238950.056.8578823
TACTATT22500.052.81217635
AGTATAG26950.048.99925633
Produced by FastQC (version 0.11.5)