FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12862802
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA791720.6155113015033583No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACAC546060.42452647564659707No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA471430.36650645792417547No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC431700.3356189421247408No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG422860.3287464115516977No Hit
CAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGCGC400490.3113551775110897No Hit
CACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCA378060.2939172973353706No Hit
GGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAA362490.2818126252740266No Hit
CCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTT356110.27685258624054077No Hit
AATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATG333560.25932141379459933No Hit
CTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAA327140.2543302773377061No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT326950.2541825645765207No Hit
GACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGC302300.23501877740168897No Hit
CACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTT293620.22827063652227564No Hit
TGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAAT261350.20318279018832755No Hit
CTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGACAAACCCGATATGGCTG230780.17941658434919547No Hit
CGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAA230150.17892679993052835No Hit
GTCTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAA214650.16687654836014734No Hit
CTGACAAACCCGATATGGCTGAGATCGAGAAATTCGATAAGTCGAAACTGAAGAAGACAGAGACGCAAGAGAAAA205840.1600273408546598No Hit
CACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGC199990.15547934268132246No Hit
GAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATT187930.14610346952398087No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG178780.13898993391953013No Hit
CCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCAT165690.12881330210944708No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTC155430.12083681300544002No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT152480.11854337802914171No Hit
CCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGACAAACCCGATATGGCTG152290.11839566526795639No Hit
CTTGTAGGCAGCAACTCACCCTCACTCAGAGGTCTTCTGGTTCTGGAAAC149320.11608668157995436No Hit
TATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAA149270.11604780980069505No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG148730.11562799458469468No Hit
CAGCAACTCACCCTCACTCAGAGGTCTTCTGGTTCTGGAAACAACTCTAGCTCAGCCTTCTCCACCATGAGCCTC145450.11307800586528502No Hit
CACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA142100.11047359665491237No Hit
GCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACAC141350.10989051996602296No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTG139980.10882543321431831No Hit
TGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGAC136330.10598779332838988No Hit
GGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGT134620.10465837847772204No Hit
ATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGC133470.10376432755475828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCAG44100.028.6137455
GTAGGCA45800.027.2457144
TGTAGGC46000.027.049693
TTGTAGG47450.026.6613642
GTTAGGG26600.026.5825211
TGCCGAC60400.025.60577870
AACGATT111550.024.97655570
CTTGTAG52400.024.2762971
CATTCGG124900.022.6458644
GCATTCG128050.022.0599083
TTCGGGC142500.020.0714176
GGAGGCT231300.019.73994670
TCGGGCC145500.019.6099957
ATTCGGG146150.019.5214885
CACTGCG140200.019.505392
ACTGCGC141000.019.493053
ACAGCAT122750.018.9181652
GAGTCGT11050.018.823525
AAACGAT104800.018.65005969
GGGCCGA154700.018.3323069
Produced by FastQC (version 0.11.5)