FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004965177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22233594
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC9261664.165615329667349No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC4636632.0854163299014994Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG4527652.036400412816749No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC4003001.800428666638421Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC3236331.455603623957512Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC2570451.156110883377649Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC2241281.0080601453818039Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2216730.9970182958274763No Hit
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC2111040.9494821215139577Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1961660.8822955029222895Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC1928070.8671877340208695Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACA1886290.8483963501357451No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA1632080.7340603592923393No Hit
TAATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACT1577900.7096918294001411Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG1415680.6367301660721159Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTTCATTCCACCGGAGTCTGAAGATCGGAAGAGCACACGTCTGAACTC1226410.5516022285915628Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG1206930.5428407121223856No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA1167350.5250388218836775Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA1156630.5202172892065944Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTCAGATCGGAAGAGCACA1147750.5162233330337866No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC1144160.5146086593107709No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC1085580.48826114212574No Hit
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG1058070.4758879738471432Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCA1025180.46109504383321925Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAAATGTTTAGGACCACTAGTAGATCGGAAGAGCACACGTCTGAACTCC1023380.460285458122515Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAAC1016560.45721802781862436Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAATGTTTAGGACCACTAGAGATCGGAAGAGCACACGTCTGAACTCCA998800.44923011547300895Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTGTTCGTTCGGCTCGCGTGAAGATCGGAAGAGCACACGTCTGAACTCC954740.4294132563543258Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC932160.4192574533833801Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA880110.39584693324884856No Hit
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC870020.3913087555705119No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC861270.3873732694768106No Hit
TACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC808930.36383231608888783Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC774540.3483647313160436No Hit
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC774360.34828377274497324Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAAGATCGGAAGAGCACAC759570.3416316768220199No Hit
GCATGGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAAC759250.3414877504734502Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC755840.3399540353215049No Hit
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT747220.33607701930691003Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTGAAATGTTTAGGACCACTAGAGATCGGAAGAGCACACGTCTGAACTCC709120.3189407884303365Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC705180.3171686952635728No Hit
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC690440.31053908783258344Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC689020.30990041466080565Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG687510.3092212622034926TruSeq Adapter, Index 12 (100% over 49bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC687200.30908183355331575Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGAAATGTTTAGGACCACTAGATCGGAAGAGCACACGTCTGAACTCCAG683030.30720629332351757Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG680110.3058929653928195Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC650840.29272820219708967No Hit
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA645600.2903714082392617Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT641550.2885498403901771No Hit
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC638590.2872185216659079Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT632520.28448841874147746Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA625510.28133553216812357Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA618930.27837604662566023Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG613710.2760282480646179No Hit
TAATACTGCCTGGTAATGATGACAGATCGGAAGAGCACACGTCTGAACTC609380.2740807446605349Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACACG582900.2621708393163966No Hit
TGAAATGTTTAGGACCACTAGATCGGAAGAGCACACGTCTGAACTCCAGT568430.2556626697420129Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACTC565880.25451575665184856Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTGTTCGTTCGGCTCGCGTGAGATCGGAAGAGCACACGTCTGAACTCCA563400.2534003274504338Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGACCTATGAATTGACAGCCATAGATCGGAAGAGCACACGTCTGAACTC552630.2485563062813866Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT546630.24585768724570575No Hit
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG543010.24422952042751164Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG500070.22491640352882222No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCAGATCGGAAGAGCACACGTCTGA497460.223742504248301Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAGTGCACTACAGAACTTTGCAGATCGGAAGAGCACACGTCTGAACTCC489270.2200588892645966Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG486040.21860613268372175No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTTGGCGCTAGATCGGAAGAGCACAC482770.2171353853092757No Hit
TGACCTATGAATTGACAGCCAGTAGATCGGAAGAGCACACGTCTGAACTC465150.20921044074115955Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC464930.20911149137651788Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCACA456520.20532892702817188No Hit
TTCACAGTGGCTAAGTTCTGCAGATCGGAAGAGCACACGTCTGAACTCCA454660.20449235512711078Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAGATCGGAAGAGCACA448800.20185670386892915No Hit
TCCTTCATTCCACCGGAGTCTGAGATCGGAAGAGCACACGTCTGAACTCC445430.20034097951055507Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAGATCGGAAGAGCA437940.19697220341434676No Hit
TAATACTGCCTGGTAATGATGAGATCGGAAGAGCACACGTCTGAACTCCA435220.19574882945150476Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGTACCGTGAGTAATAATGCAGATCGGAAGAGCACACGTCTGAACTCCA409620.18423472156593307Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTCAGATCGGAAGAGCACACGTCTGAACTCC408400.1836860023620113Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG399040.17947615666634914No Hit
TTCACAGTGGCTAAGTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT396040.1781268471485087Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG381750.17169963614519543Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCACATTGCCAGGGATTACCACTAGATCGGAAGAGCACACGTCTGAACT375250.16877613218987447Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAAGATCGGAAGAGCACACG374280.16833985544577273No Hit
TTCAAGTAATCCAGGATAGGCAAGATCGGAAGAGCACACGTCTGAACTCC371930.16728289632346438Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATACTGCCGGGTAATGATGGAGATCGGAAGAGCACACGTCTGAACTCC366690.16492610236563643Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA365990.16461126347814034TruSeq Adapter, Index 12 (100% over 40bp)
TCCTTCATTCCACCGGAGTCTGTAGATCGGAAGAGCACACGTCTGAACTC360850.16229944650424039Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTTCTGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC355340.15982121468980678Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT353250.1588811957257113Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAAGCGGGTGCTCTTATTTTAGATCGGAAGAGCACACGTCTGAACTCCAG346290.15575079764432148Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA337170.15164889671008655Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGCTCAGTTCAGCAGGAACAGATCGGAAGAGCACACGTCTGAACTCCAG327670.14737608323692516Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTTCCGTAGTGTAGTGGTCATCAGATCGGAAGAGCACACGTCTGAACTC325930.1465934837165777Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGATCGGAAGAGCACA323800.145635473958911No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT317700.1428918779393021Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGGACTTGGAGTCAGAAGGCAGATCGGAAGAGCACACGTCTGAACTCC308450.13873150692596076Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG303620.13655911860223768No Hit
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT301090.1354212009088589TruSeq Adapter, Index 1 (97% over 35bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTAGATCGGAAGAGCACA300390.1351063620213628No Hit
TGGCTCAGTTCAGCAGGAACAGAGATCGGAAGAGCACACGTCTGAACTCC295520.1329159829040685Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAGATCGGAAGAGCAC291560.13113489434051914No Hit
TCCCTGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAAC290790.1307885715642734Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCACATGGTCTAGCGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA286840.12901198069911685Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC286750.12897150141358163Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGGAAGAGCACACGTC281100.1264303018216488No Hit
AGCTACATCTGGCTACTGGGTCTCTAGATCGGAAGAGCACACGTCTGAAC274090.123277415248295Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAATACTGCCTGGTAATGATGACAAGATCGGAAGAGCACACGTCTGAACT273360.12294908326562048Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAAGCGGGTGCTCTTATTTAGATCGGAAGAGCACACGTCTGAACTCCAGT272280.12246333183919794Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTTGGCGCTCAGATCGGAAGAGCACA264520.11897311788638401No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC262730.11816802987407253No Hit
AACCCGTAGATCCGATCTTGTGAAGATCGGAAGAGCACACGTCTGAACTC260430.1171335592437282Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC259200.11658034234141362No Hit
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC253540.11403464505108801Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTGTTCGTTCGGCTCGCGTGTAGATCGGAAGAGCACACGTCTGAACTCC249890.11239298513771548Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT249810.11235700355057307No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC248860.11192972220325692No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACAC247260.1112100904604087No Hit
TGAGGTAGTAGATTGTATAGTCAGATCGGAAGAGCACACGTCTGAACTCC243910.10970336149882022Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGATCTTGTGAGATCGGAAGAGCACACGTCTGAACTCC242440.1090421998350784Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATACTGCCGGGTAATGATGAGATCGGAAGAGCACACGTCTGAACTCCA239110.10754446627027552Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTAGATCGGAAGAGCACACGTCTGAACTCC238700.10736006063617065Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTTGGCGCTAGATCGGAAGAGCACACG238160.10711718492295937No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTAGATCGGAAGAGCACACGTCT232980.10478737715548822Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT231650.10418918326924563No Hit
TGAGGTAGTAGTTTGTGCTGTCAGATCGGAAGAGCACACGTCTGAACTCC231490.1041172200949608Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTC230380.10361797557335985Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAATACTGCCTGGTAATGATGACCAGATCGGAAGAGCACACGTCTGAACT229090.10303777248068845Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTGACCTATGAATTGACAGCCAGATCGGAAGAGCACACGTCTGAACTCCA223970.10073495090357411Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)