FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25657506
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG370030.14421900554169215No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG300750.11721716054553394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG292850.030.6071321
TATCGCG16150.021.0815499
GTGCCGA44700.019.82254626
CGTATCG24200.019.8117493
GTATCGA23900.019.7696254
TTACTCG17200.018.38807535
TCGTATG15350.018.34703845
TGGGGGG288300.017.38351
GCCGAAA50900.017.20502528
TATCGAA28650.016.9769275
CGCGTAT4550.016.0407411
CCCGTAT34250.016.0329361
GATCGGA20900.014.9662421
GACCACG86650.014.85709470
CCGTATC35100.014.5504672
CGTATGC20100.014.35772946
CGTTCCG34900.014.24125836
TATGCCG20900.014.14167748
AGTGCCG65100.014.037497525
CTCCGAA63000.013.79384839
Produced by FastQC (version 0.11.5)