FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25657506
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1435470.5594737072262597No Hit
CTCGGCTTTGACAGAGTGCAAGACGATGACTTGCAAAATGTCGCAGCTGG285320.11120332584156854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCTT107850.021.4640772
CTCGGCT119000.020.999461
CGGCTTT112450.020.0003383
TATCGCG19400.019.68479
TGTCGCA117200.018.43035339
AATGTCG120400.018.14301537
CGATGAC126850.017.35534524
GACGATG127200.017.33431222
ACGCAAC124700.017.30557352
GTCGCAG130700.016.60684840
ACGATGA133750.016.58929623
TCGCAGC133600.016.35082441
AAGACGA136450.016.3119320
ATGTCGC137750.015.80730438
AACGCAA144250.015.10344751
AGACGAT151550.014.98463221
CTCGACT43650.014.7894261
CGCAACA150450.014.71498353
TTGACAG157400.014.3584428
CGCAGCT155100.014.35352742
Produced by FastQC (version 0.11.5)