FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25562348
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2185490.8549644970016056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG30200.025.0661099
CGACGTA18100.017.07834222
GACCTAG61150.016.6933065
TAACGGG15350.016.61575969
ATCGCGG48300.016.03258710
CTAACGG15050.015.96570168
GATATCG48900.015.8349377
CGACCGT19550.015.6263521
ACTAACG15450.015.44541567
CTCGACG20550.014.87313720
TCGCGGC53500.014.86462411
TACGTTC18600.014.5576292
CGACCTA15100.014.4853684
GTTACGG59250.014.450294559
ATATCTG108100.014.34251770
TTACGGT59700.014.17092860
TGTTACG59650.014.11726458
CCTAACT83600.013.7065467
CGCTCGA22400.013.64410718
TCGAAGT28550.013.57640870
Produced by FastQC (version 0.11.5)