FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25988239
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG367190.1412908354429094No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG298930.1150251080883164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG308650.033.652141
CGTATCG29250.020.3013573
TGGGGGG330150.020.0864141
GTATCGA29300.020.0296754
TATCGCG13900.019.735459
TCGTATG13300.018.03209145
TATCGAA35250.018.0277965
CCCGTAT45150.015.3109851
CCGTATC42500.014.952842
AGATCGG31700.013.5836744
ATCGCGC42950.013.42351467
CGTTCCG27150.013.30721636
TATGCCG18200.013.17993648
GATCGGT31550.013.0977975
CGTATGC20500.012.88665346
ATCGGTC33250.012.3236976
ATCGAAG57850.012.3055536
TCGACGT12150.012.28983821
CGTGTTA46500.012.13493959
GTTCCGA28350.012.13184237
Produced by FastQC (version 0.11.5)