FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25988239
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3926641.5109296170471573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG15250.017.0671069
TCGGCTT79800.016.0422632
TAGGTCG12600.015.6954313
CTCGGCT94450.015.2076851
CGGCTTT82400.014.6107053
TGTCGCA80900.013.98907439
AATGTCG82000.013.92842237
ACGCAAC82500.013.64691652
ATGTCGC86950.013.25541638
TACCGGT14200.012.95978444
GTCGCAG89000.012.83327540
AGACGAT97550.012.48912221
ACCGGTA15500.012.32174545
TCGCAGC95250.012.10086441
CGATGAC97150.011.93353724
GACGATG99300.011.84945622
CTCGACT38700.011.4752921
AACGCAA103650.011.36355951
ACGATGA108250.010.77374823
GCGATAG31350.010.70970966
Produced by FastQC (version 0.11.5)