FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004965187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20470598
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC11328435.534000521137682No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC9785324.780182777269134No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG7309093.5705307680801512No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG4737092.314094585805456No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT3642051.7791615076413496No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG3351011.6369868628166115No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG2412031.178289955183527No Hit
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA2095811.0238147415136578Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG1784170.8715768830983834No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC1639000.8006605376159505Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA1611380.7871680153164066No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC1495810.7307114330514428No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1367950.6682511180181449No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG1331060.6502301496028597No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC1320240.6449445199402578No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC1312440.6411341769302489No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC1225200.5985169558798429No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1141420.5575899639082356No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC1123760.5489629565291645Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC1120960.5475951410896741No Hit
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC1090800.5328618147843067No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC1077320.5262767604541889Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG985680.4815101151417267No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT905620.4424003636825852No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG844140.4123670446754902No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC802320.39193774407567383No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC782900.3824509669917801No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAAGATCGGAAGAGCACAC769160.37573890122799536No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC745800.36432741241853317No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT706360.34506075494228355No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC703580.3437027096130753Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCA697460.3407130558667607Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATCGGAAGAGCAC669010.3268150739905107No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC651150.31809036550861874No Hit
TTTCTGTGTGGAATTTGAATATCTGAAAAGATCGGAAGAGCACACGTCTG649110.31709381425984723Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG599940.2930739981313687Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC596870.29157428620307035Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCAGATCGGAAGAGCACA575970.2813645209583032No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCACA546790.2671099300567575No Hit
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC545290.26637717178560194Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACACG532970.26035878385184447No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA499530.24402315946021702No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC489330.2390404032163594No Hit
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC484350.23660764575612303Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG484230.23654902509443057No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAGATCGGAAGAGCA472400.23077000486258387No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTCAGATCGGAAGAGCACA447070.2183961601903374No Hit
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACACGTCTGAACTC430460.21028208360107506Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCAGTGATGACCCTCATCTATCACCCTTGACTGATGAGATCGGAAGAGC422910.20659386696959217No Hit
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC402170.19646226260708163Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGAGATCGGAAGAGCACACGTCTGAACTCC401140.19595910192755484Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTAATGATGAATGCCAACCGCTCTGATGAGATCGGAAGAGCACACGTCT398400.19462059681891072Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAA397340.1941027809739608No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG388780.1899211737732332No Hit
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAAC380210.18573468151736455Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC377670.1844938775115412No Hit
TTACTTGATGACAATAAAATATCTGATAAGATCGGAAGAGCACACGTCTG376470.18390767089461676Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG374060.18273037260562686No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG366160.17887117904420768Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATG346790.1694088272360192TruSeq Adapter, Index 1 (97% over 37bp)
TACCCTGTAGAACCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC344490.1682852645535807Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC343590.16784560959088737Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGAT342520.16732290869079644No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT340210.16619446095321688No Hit
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC339300.16574992093538254Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACA336420.1643430250547639No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC335790.16403526658087858No Hit
AACCCGTAGATCCGATCTTGTGAAGATCGGAAGAGCACACGTCTGAACTC333270.16280423268533728Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGACTGACCTGAAATGAAGAGAATACTCATTGCTGATCAGATCGGAAGA332500.16242808343947746No Hit
ACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCAGATCGGAAGAGCA324610.15857377493319932No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAAGATCGGAAGAGCACACG314270.15352262791736715No Hit
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT308910.15090423836177136Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGGAAGAGCACACGTC302780.14790969956031572No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACG299460.14628786125349147No Hit
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC296860.14501774691682187Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG291660.14247751824348268No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC287930.1406553926758759No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTAGATCGGAAGAGCACACG282920.13820798005021642No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACA282620.13806142839598531No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGATCGGAAGAGCA281180.137357980455676No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTC279880.1367229232873412No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGT277270.13544792389553056No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACAC272450.13309332731755077No Hit
ACCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC262740.1283499387756039No Hit
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA259240.12664016947624102Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC258520.12628844550608634Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGAGATCGGAAGAGCAC256290.1251990782096351No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGGAAGAGCACACG254530.1243393085048126No Hit
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA252920.12355281462710567Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTTCATTCCACCGGAGTCTGAAGATCGGAAGAGCACACGTCTGAACTC251410.1228151713008091Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGAGATCGGAAGAGCACACGTCT241340.11789592077378491Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA239010.11675770292592332No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGAAGAGCACACG238080.1163033927978069No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC237310.11592724355194703No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT235500.11504304857141935No Hit
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT233600.11411488809462235Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAAGATCGGAAGAGCACACG232570.11361172741509555No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAGATCGGAAGAGCAC232550.11360195730481346No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA231180.11293270475049141Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTAGATCGGAAGAGCACA226270.11053414267624229No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG216880.10594707589880863No Hit
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC216850.1059324207333855Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT214220.1046476512312928No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGAGATCGGAAGAGCACACG210810.10298184742819921No Hit
TAATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACT210190.10267897400945492Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGGGTGATGCGAACTGGAGTCTGAGCAGATCGGAAGAGCACACGTCTGA206760.10100340009607926Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG206220.10073960711846326Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT206030.10064679107078357No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)