FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22239806
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC22137239.95387729551238No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG9386384.220531420103215No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT4752182.136790222001037No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC3771851.6959905135863145Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA3598261.6179367751679128Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC3182011.4307723727446184Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG2837011.2756451202856716No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACA2655741.1941381143342706No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC2482551.1162642335998794No Hit
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC2054160.9236411504668701No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC1589320.7146285358784155Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC1519890.6834097383763149No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1472180.6619572131159778No Hit
TCCCTGGTAGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC1465580.6589895613298066No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAAGATCGGAAGAGCACAC1462740.6577125717733329No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC1392330.626053122945407No Hit
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1358440.6108146806676281Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC1306750.5875725714513877No Hit
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1253620.5636829745727099Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG1138380.5118659758093214Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC1114870.5012948404316117No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACACG1090680.4904179469910844No Hit
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC1037170.4663574853125967Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC1032870.46442401520948523Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG976160.4389246920589145Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG971870.4369957183979033No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC963350.43316474972848235Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAAC955600.4296800071007813Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT940400.42284541510838713No Hit
ATGACCTATGAATTGACAGACAAGATCGGAAGAGCACACGTCTGAACTCC895480.40264739719402226Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACT894320.4021258099103922Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAGATCGGAAGAGCAC882120.39664015054807583No Hit
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC879730.3955655008861138Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG852930.3835150360574188No Hit
CTGACCTATGAATTGACAGCCATAGATCGGAAGAGCACACGTCTGAACTC801920.360578684903996Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC797060.35839341404326996Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA782610.35189605520839523No Hit
TGACCTATGAATTGACAGCCAGTAGATCGGAAGAGCACACGTCTGAACTC759150.34134740204118685Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCA744210.33462971754339943Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC737180.33146871874691713No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG719400.323474044692656No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC690570.3105108021176084No Hit
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC686060.30848290673039147Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG683940.30752966100513646No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG679790.3056636375335289No Hit
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC638870.28726419645926765Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA613550.27587920506141106Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAAGATCGGAAGAGCACACG611910.2751417885569685No Hit
TACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC602800.2710455298036323Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC597370.26860396174319146Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC563800.2535094056126209No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC506360.22768184218873133No Hit
ACCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC491760.2211170367223527No Hit
CTGACCTATGAATTGACAGCCAGATCGGAAGAGCACACGTCTGAACTCCA487250.21908914133513574Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC480320.21597310695965605Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC457980.2059280553076767Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC441040.19831108238983738No Hit
TCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT435940.19601789691870514No Hit
TTTCTGTGTGGAATTTGAATATCTGAAAAGATCGGAAGAGCACACGTCTG432410.1944306528573136Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAATACTGCCTGGTAATGATGACAGATCGGAAGAGCACACGTCTGAACTC429390.19307272734303527Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTACCGTGAGTAATAATGCAGATCGGAAGAGCACACGTCTGAACTCCA415600.18687213368677766Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA415380.18677321196057195Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGACCTATGAATTGACAGCCAGAGATCGGAAGAGCACACGTCTGAACTCC411380.18497463512046822Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT410990.18479927387855813Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT391820.176179594372361No Hit
TCCCTGTAGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG390450.17556358180462547No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG387100.17405727370103857Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA386360.1737245369856194Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATCGGAAGAGCAC381970.17175059890360553No Hit
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCAGATCGGAAGAGCACA375750.16895381191724426No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTAGATCGGAAGAGCACACGTCT374380.1683377993495087Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCCGTAGATCCGATCTTGTGAAGATCGGAAGAGCACACGTCTGAACTC370320.16651224385680344Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACG363890.16362103158633667No Hit
AATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACTC361250.1624339708718682Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC359070.16145374649401167No Hit
TCAGTGCACTACAGAACTTTGCAGATCGGAAGAGCACACGTCTGAACTCC351610.15809940068721823Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTAATGATGAATGCCAACCGCTCTGATGAGATCGGAAGAGCACACGTCT348550.15672348940453887Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCGAGATCGGAAGAGCACAC346990.1560220444368984No Hit
TCCCACATGGTCTAGCGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA341100.1533736400398457Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAGATCGGAAGAGCA336340.15123333360012223No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACAC334700.1504959170956797No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCAGATCGGAAGAGCACACGTCTGA327420.14722250724669092Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAGATCGGAAGAGCACA323390.14541044108028642No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT322490.14500576129126305No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT317970.14297336946194583No Hit
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC313670.14103989935883432Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT302680.13609830949064933Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAAGATCGGAAGAGCACACG301840.13572060835422756No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGATCGGAAGAGCACA293400.13192561122160867No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT287040.12906587404584374No Hit
TGACCTATGAATTGACAGCCAAGATCGGAAGAGCACACGTCTGAACTCCA281550.12659732733280138Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT274250.12331492459961206Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA271020.12186257380122831Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGATCGGAAGAGCA268900.12090932807597332No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCACA268050.12052713049745128No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC266300.1197402531299059No Hit
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG265800.11951543102489293Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC265360.11931758757248152Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCAAGATCGGAAGAGCACACGTCTGAACTCC251570.11311699391622392Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC247340.11121499890781421No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA239080.10750093773300001No Hit
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA236860.10650272758674245Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG233690.10507735544096022No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA229470.1031798568746508No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTAGATCGGAAGAGCAC227410.10225358980199736No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCAGATCGGAAGAGCACACGTCTGA222460.100027850962369Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTGG3939800.069.948881
ATTGGTG2148600.069.869553
GTTTCCG593000.069.868871
GCATTGG2144300.069.826821
TCCCTGT1580900.069.766191
AGCTTAT432900.069.738722
GATAGGC569250.069.73048415
TTGGTGG2175300.069.728164
ATAGGCT526800.069.7025416
TCCCACA198400.069.698871
TAGTGTA663950.069.692518
CCCTGGT4100400.069.669222
TTTCCGT595200.069.664462
GCTTATC433900.069.657893
AAGTAAT623450.069.6512154
CCAGGAT572150.069.6378811
GTTCGCC395200.069.61233526
CCCACAT202500.069.607992
TAATCCA570600.069.606577
CGTAGTG598850.069.601626
Produced by FastQC (version 0.11.5)