FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004965192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20265515
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC6180983.0499989760931316No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC5341682.6358471521695845Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC5264952.5979848032482766Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC5059842.4967734597418323Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA3934941.9416925748000975Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCA3266361.6117823800678148Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC3202291.5801670966664307Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG2956911.4590845581767846Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG2840201.4014941145093032No Hit
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC2238281.1044772363297948Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA1940780.9576761311025158Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1844310.9100730970814214No Hit
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1807170.8917463977599386Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC1768920.8728719699450027Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1625940.8023186185991327Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG1304700.6438030318992634No Hit
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC1194800.5895729765564803Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC1139690.5623789970301767Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC1137090.5610960293878542Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGATCTTGTGAAGATCGGAAGAGCACACGTCTGAACTC1104800.545162558168396Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAAC962250.47482138993260226Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG939240.4634671262980487Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT895600.4419330078707598Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC785690.38769801803704473Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT778200.38400208432896965Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCTGTGTGGAATTTGAATATCTGAAAAGATCGGAAGAGCACACGTCTG770030.379970605237518Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG751470.3708121900677086Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC740950.36562110560723476Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTACCGTGAGTAATAATGCAGATCGGAAGAGCACACGTCTGAACTCCA737930.3641308893457679Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG715040.35283583960239845No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG642410.31699663196321437Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACCCTGTAGAACCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC630920.3113269018823356Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC611650.30181813785635353No Hit
TGAGAACTGAATTCCATAGGCTGAGATCGGAAGAGCACACGTCTGAACTC596920.2945496327135037Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC590470.29136688606235767Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC581190.28678767847745296Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC576470.28445859875754453Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC566060.27932179369732274Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC565940.27926257980613867No Hit
TGGAAGACTAGTGATTTTGTTGTTAGATCGGAAGAGCACACGTCTGAACT558140.27541367687917134Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACCCGTAGATCCGATCTTGTGAGATCGGAAGAGCACACGTCTGAACTCC538640.26579141956175306Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA524280.2587054905833876Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC515450.2543483350904233No Hit
TCCTTCATTCCACCGGAGTCTGAAGATCGGAAGAGCACACGTCTGAACTC501480.24745485125840622Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGTGCACTACAGAACTTTGCAGATCGGAAGAGCACACGTCTGAACTCC493980.2437539830593992Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC486960.24028997042512862No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG467710.23079107538101054No Hit
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG464280.22909854499133134Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA461090.22752444238402034Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTAATGATGAATGCCAACCGCTCTGATGAGATCGGAAGAGCACACGTCT458730.22635990252406613Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCACAGTGGCTAAGTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT456200.22511147631826775Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA446810.22047798933311097Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC445710.21993519533058992No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA444640.21940720480086492Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC436030.21515860810840484No Hit
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACACGTCTGAACTC416940.20573866491919893Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG416330.20543766097234636No Hit
TCGTACCGTGAGTAATAATGAGATCGGAAGAGCACACGTCTGAACTCCAG414020.20429779356705222Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA413150.2038684928559674Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC401570.19815435235670054Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGGGTGATGCGAACTGGAGTCTGAGCAGATCGGAAGAGCACACGTCTGA381690.1883445843838659Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT380120.1875698693075404No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGGAAGAGCACACGTC378290.1866668574669827No Hit
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA372210.183666686980321Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCAGATCGGAAGAGCACACGTCTGAACTCCA371410.18327192770576026Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGAGATCGGAAGAGCACACGTCTGAACTCC368650.18191000820852568Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACAGTAGTCTGCACATTGGTTAGATCGGAAGAGCACACGTCTGAACTCCA364670.17994608081758592Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACT364560.17989180141733382Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAAGATCGGAAGAGCACAC355480.1754112836510693No Hit
TAATACTGCCTGGTAATGATGACAGATCGGAAGAGCACACGTCTGAACTC347340.1713946080324137Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGATGAAGCACTGTAGCCAGATCGGAAGAGCACACGTCTGAACTCCAG342370.16894216603920503Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC339160.16735819445003003Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGATCTTGCAGATCGGAAGAGCACACGTCTGAACTCCA336400.16599627495279543Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACA324380.16006501685252014No Hit
TTACTTGATGACAATAAAATATCTGATAAGATCGGAAGAGCACACGTCTG324260.16000580296133604Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT323770.1597640129056676Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCAAGTAATCCAGGATAGGCAAGATCGGAAGAGCACACGTCTGAACTCC316880.1563641486535131Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG309230.15258926309052595No Hit
GTTTCCGTAGTGTAGTGGTCATCAGATCGGAAGAGCACACGTCTGAACTC309040.15249550776281776Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTAGATCGGAAGAGCACACGTCTGAACTCC307110.15154315101293997Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCACATGGTCTAGCGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA303790.14990490002351284Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAAATGTTTAGGACCACTAGAGATCGGAAGAGCACACGTCTGAACTCCA298490.14728961982954789Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTCAGATCGGAAGAGCACACGTCTGAACTCC297930.14701328833735536Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG296140.14613001446052568No Hit
TGGCTCAGTTCAGCAGGAACAGATCGGAAGAGCACACGTCTGAACTCCAG295190.14566123782198478Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA294960.14554774453054858No Hit
GTGAAATGTTTAGGACCACTAGAGATCGGAAGAGCACACGTCTGAACTCC291690.1439341659957815Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGAACTGAATTCCATAGGCTGTAGATCGGAAGAGCACACGTCTGAACT290830.14350979977562872Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAAATGTTTAGGACCACTAGTAGATCGGAAGAGCACACGTCTGAACTCC290350.14327294421089223Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG289860.14303115415522377No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACACG289520.14286338146353547No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAGATCGGAAGAGCAC289340.1427745606267593No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATCGGAAGAGCAC283580.1399322938499219No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTAGATCGGAAGAGCACACGTCT282700.13949805864790507Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC278270.13731207916502494Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCAGATCGGAAGAGCACA275700.1360439149954985No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC270880.13366549036626998No Hit
TTCAAGTAATTCAGGATAGGTTAGATCGGAAGAGCACACGTCTGAACTCC267050.13177558033931042Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGCTCAGTTCAGCAGGAACAGAGATCGGAAGAGCACACGTCTGAACTCC265620.13106994813603307Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTAGATCGGAAGAGCACACGTCTGAACTCCA265470.13099593077205293Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC259580.1280895156130994No Hit
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC256810.12672266162493281Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGAAAGATCGGAAGAGCACACGTCTGAACTC252780.12473406177933302Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTAAGATCGGAAGAGCACACGTCTGAACTC247440.12209904362164002Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC247430.122094109130708Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC236600.11675005545134184Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCAGATCGGAAGAGCACACGTCTGA236050.11647865845008133Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC232070.1145147310591416No Hit
TAACACTGTCTGGTAACGATGAGATCGGAAGAGCACACGTCTGAACTCCA230350.11366599861883599Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAATACTGCCTGGTAATGATGAGATCGGAAGAGCACACGTCTGAACTCCA221390.10924469474375559Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA220880.10899303570622311No Hit
TGAGAACTGAATTCCATGGGTTAGATCGGAAGAGCACACGTCTGAACTCC218760.10794692362863711Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC218360.10774954399135675No Hit
TAGCTTATCAGACTGATGTTAGATCGGAAGAGCACACGTCTGAACTCCAG217540.10734491573493198Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTACTTGATGACAATAAAATATCTGATAGATCGGAAGAGCACACGTCTGA216540.10685146664173105Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGACCTATGAATTGACAGCCATAGATCGGAAGAGCACACGTCTGAACTC212550.10488260475985929Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATCTCGTATG211440.10433487626640625TruSeq Adapter, Index 25 (100% over 49bp)
TGAGATGAAGCACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC211240.10423618644776608Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG210490.10386609962786536No Hit
AACCCGTAGATCCGATCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG208340.10280518407748335Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATA207710.10249431114876675TruSeq Adapter, Index 25 (100% over 40bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGT202720.10003200017369407No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)