FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004965199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19089628
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG868591545.50070331386238No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC7519343.938966228152796No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT5545372.9049125525128097No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG4969762.603382318398242No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG4751492.4890427409062132No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGAAGAGCACACG3800261.9907459694866763No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2915751.5274001148686605No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA2187621.145973090727593No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAGATCGGAAGAGC1432340.7503236836254745No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC1243460.651379901169368Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC589460.3087854828810703Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC546760.2864173152038374Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAAGATCGGAAGAGCACA539250.2824832416849611No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTAGATCGGAAGAGC534220.27984830296326363No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGCGCACACG508390.2663173949749047No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC491060.25723916673494107Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT479360.251110184022444No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTC454800.23824455877296302Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC428530.2244831591270401Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAAGATCGGAAGAGCACAC415280.21754221716630626No Hit
GGCCGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG377140.19756278121291834No Hit
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGCACACGTCTGA367470.19249720319327335Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAAGATCGGAAGAG355340.1861429672699751No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTATATCGGAAGAGCACACG349050.18284798425616255No Hit
GGCTGGTCCGATGGTAGTGGGTTATCGGAACTAGATCGGAAGAGCACACG348250.18242890851513713No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTCAGATCGGAAGAGCACAC343730.1800611305783434No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAACAGATCGGAAGA328350.17200439945712928No Hit
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC306730.16067887755591675Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTAGATCGGAAGAGCACA297650.155922367895278No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGCTCGGAAGAGCACACGT275540.14434016210268738No Hit
GGCTGGCCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG270500.1416999849342271No Hit
GGGTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG259320.13584340145339657No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGGACTAGATCGGAAGAGCACACG236490.12388402749388305No Hit
AGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG234290.12273156920606311No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC233960.12255870046289012Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGGTGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG232770.12193532529811477No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAACTAGATCGGAAG216360.11333903416033042No Hit
GGCTGGTCCGATGGTAGTGGGTTACCAGAACTAGATCGGAAGAGCACACG213860.11202942246962592No Hit
GGCTGGTCCGATGGTGGTGGGTTATCAGAACTAGATCGGAAGAGCACACG206880.10837298662917895No Hit
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA191700.10042102444322121Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)