FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11881128
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT763510.6426241683449585No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT406050.3417604793080253No Hit
CTCCACTTTTTCAATTCTCTCTCCATTCTTCAGTAAGTCAACTTCAATGTCGGATGGATGAAACCCAGACACAT404210.34021180480506563No Hit
CTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCT365830.30790847468354854No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT351170.29556957891540264No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTC343600.2891981300092045No Hit
CACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACA298750.251449188999563No Hit
CTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGG296010.24914301066363398No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC282840.23805820457451515No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA282450.23772995291356175No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT279250.2350366059518928No Hit
CTTGTGGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCG245640.20674804614511352No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC234300.1972034978496991No Hit
GGCAATGCTTGTGGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTA225910.1901418787845733No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT224010.1885427040260824No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG220160.18530227096282442No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGC197550.16627209133678217No Hit
CGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGTCTCTGTCTTCTTC196140.16508533533179678No Hit
GCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTT193710.16304007498277942No Hit
GTGGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCT189660.15963130773441714No Hit
CTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA178960.15062542883133653No Hit
GCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATT174480.14685474308500002No Hit
GGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGAC170220.1432692249422782No Hit
CTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTA159610.13433909642249456No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG157390.13247058696783673No Hit
CTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGG149620.12593080387653427No Hit
GTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATT134060.11283440427541896No Hit
GGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATC130790.1100821403489635No Hit
CCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTT129320.10884488408844682No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG128220.10791904607037311No Hit
GTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGA123260.10374435827978623No Hit
GGTGAATTCAGTGTAGTACAAGAGATAGAAAGACCAGTCCTTGCTGAAAGACAAGTCTGAATGCTCCACTTTTTC122930.10346660687436413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATCG126750.032.61755870
ATGTCGG114550.030.2793847
AATGTCG115400.030.14285546
GTCGGAT117250.029.58722549
TGTCGGA118600.029.21802148
CGCGCAA854.1679014E-628.6375471
TCGGATG125950.027.68406750
AGTCAAC128700.026.90807736
GTCAACT130350.026.48837
AGTAAGT135100.025.81092332
CGGATGG137150.025.50115651
GTAAGTC136350.025.44756133
AAACCCA138000.025.22817661
ACCCAGA136850.025.1189863
AACCCAG138550.025.03142762
GACACAT142650.024.8334168
CCCAGAC139750.024.80190864
GATGGAT141050.024.62801653
CAGTAAG144400.024.19653731
ACTTATC134550.023.72198169
Produced by FastQC (version 0.11.5)